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Issue 1, 2012
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On-chip sample preparation by controlled release of antibodies for simple CD4 counting

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Abstract

We present a simple system for CD4 and CD8 counting for point-of-care HIV staging in low-resource settings. Automatic sample preparation is achieved through a dried reagent coating inside a thin (26 μm) counting chamber, allowing the delayed release of fluorochrome conjugated monoclonal antibodies after the filling of the chamber with whole blood by capillary flow. A custom-built image cytometer is used to capture fluorescence images representing more than 1 μl of blood. The thin layer of blood in combination with the large image area allows the use of whole blood from a finger prick without the need for dilution, lysis or cell enrichment. Automatic cell counting of CD4+ and CD8+ T-lymphocytes correlates well with results obtained by flow cytometry.

Graphical abstract: On-chip sample preparation by controlled release of antibodies for simple CD4 counting

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Article information


Submitted
28 Jun 2011
Accepted
06 Oct 2011
First published
03 Nov 2011

Lab Chip, 2012,12, 167-173
Article type
Paper

On-chip sample preparation by controlled release of antibodies for simple CD4 counting

M. Beck, S. Brockhuis, N. van der Velde, C. Breukers, J. Greve and L. W. M. M. Terstappen, Lab Chip, 2012, 12, 167
DOI: 10.1039/C1LC20565J

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