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Issue 5, 2012
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Fish and Chips: a microfluidic perfusion platform for monitoring zebrafish development

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Abstract

We have developed a multi-channel microfluidic perfusion platform for culturing zebrafish embryos and capturing live images of various tissues and organs inside the embryo. The Fish and Chips was micro-fabricated in silicon and glass for reproducibility and accuracy of the microfluidic structure. The microfluidic platform consists of three parts: a microfluidic gradient generator, a row of eight fish tanks, in which the fish embryos are individually placed, and eight output channels. The fluidic gradient generator supports dose-dependent drug and chemical studies. A unique perfusion system ensures a uniform and constant flow of media to the fish tank while the wastes are efficiently removed. The fish tanks restrict the embryo movements, except rotationally, for live imaging of internal tissues and organs. The embryos showed developmental abnormalities under the influence of the drug valproic acid (VPA).

Graphical abstract: Fish and Chips: a microfluidic perfusion platform for monitoring zebrafish development

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Article information


Submitted
26 Apr 2011
Accepted
08 Nov 2011
First published
07 Dec 2011

Lab Chip, 2012,12, 892-900
Article type
Paper

Fish and Chips: a microfluidic perfusion platform for monitoring zebrafish development

D. Choudhury, D. van Noort, C. Iliescu, B. Zheng, K. Poon, S. Korzh, V. Korzh and H. Yu, Lab Chip, 2012, 12, 892
DOI: 10.1039/C1LC20351G

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