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Issue 24, 2011
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Mass sensors with mechanical traps for weighing single cells in different fluids†

Yaochung Weng,‡ab   Francisco Feijó Delgado,‡bc   Sungmin Son,d   Thomas P. Burg,e   Steven C. Wassermanb  and  Scott R. Manalis*bc  
Author affiliations

* Corresponding authors

a Computational & Systems Biology Initiative, Massachusetts Institute of Technology, Cambridge, MA, USA

b Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA
E-mail: scottm@media.mit.edu

c Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA

d Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA

e Max Planck Institute for Biophysical Chemistry, Göttingen, Germany

Abstract

We present two methods by which single cells can be mechanically trapped and continuously monitored within the suspended microchannel resonator (SMR) mass sensor. Since the fluid surrounding the trapped cell can be quickly and completely replaced on demand, our methods are well suited for measuring changes in cell size and growth in response to drugs or other chemical stimuli. We validate our methods by measuring the density of single polystyrene beads and Saccharomyces cerevisiae yeast cells with a precision of approximately 10−3 g cm−3, and by monitoring the growth of single mouse lymphoblast cells before and after drug treatment.

Graphical abstract: Mass sensors with mechanical traps for weighing single cells in different fluids

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Article information

https://doi.org/10.1039/C1LC20736A
Submitted
09 Aug 2011
Accepted
07 Oct 2011
First published
28 Oct 2011
Lab Chip, 2011,11, 4174-4180
Article type
Paper
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Mass sensors with mechanical traps for weighing single cells in different fluids

Y. Weng, F. F. Delgado, S. Son, T. P. Burg, S. C. Wasserman and S. R. Manalis, Lab Chip, 2011, 11, 4174
DOI: 10.1039/C1LC20736A

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