Jump to main content
Jump to site search

Issue 24, 2011
Previous Article Next Article

Patterning cells and shear flow conditions: Convenient observation of endothelial cell remoulding, enhanced production of angiogenesis factors and drug response

Author affiliations

Abstract

We present a method that allows patterning cells and shear flow conditions for endothelial cell based assays. This method is novel in combining (1) cell culture on the surface of a substrate both topographically and chemically patterned; (2) multi-shear flow assays after covering the cell substrate with a microfluidic cover plate containing microchannels of different channel widths, and (3) conventional immunostaining assays after removal of the cover plate. This method has the advantage of performing cell cultures and immunoassays in standard cell biology environments with open access, facilitating the formation of confluent cell layers and the observation of cell responses to shear-flow and drug stimulations. To obtain multi-shear stress conditions, a single channel with stepwise increasing channel widths was patterned on the surfaces of both the substrate and the microfluidic cover plate. As results, we observed excellent viability of endothelial cells in the whole range of applied shear stresses (0–25 dyn cm−2) and shear stress dependent cytoskeleton remoulding, activation of von Willebrand factor (vWF), and re-organisation of angiogenesis factors such as tetra peptide acetyl-Ser-Asp-Lys-Pro (AcSDKP) of endothelial cells. To validate this approach for drug analysis, we also studied drug effects under shear stress conditions. Our results indicate that the drug effect of combretastatin A-4, an anti-tumour vascular targeting drug, could be significantly enhanced under shear flow conditions.

Graphical abstract: Patterning cells and shear flow conditions: Convenient observation of endothelial cell remoulding, enhanced production of angiogenesis factors and drug response

Back to tab navigation

Publication details

The article was received on 05 Aug 2011, accepted on 09 Sep 2011 and first published on 03 Nov 2011


Article type: Paper
DOI: 10.1039/C1LC20722A
Lab Chip, 2011,11, 4235-4240

  •   Request permissions

    Patterning cells and shear flow conditions: Convenient observation of endothelial cell remoulding, enhanced production of angiogenesis factors and drug response

    L. Wang, Z. Zhang, J. Wdzieczak-Bakala, D. Pang, J. Liu and Y. Chen, Lab Chip, 2011, 11, 4235
    DOI: 10.1039/C1LC20722A

Search articles by author

Spotlight

Advertisements