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Issue 13, 2011
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Development and characterization of a microfluidic chamber incorporating fluid ports with active suction for localized chemical stimulation of brain slices

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Abstract

We report a novel microfluidic chamber incorporating fluid ports with active suction to achieve localized chemical stimulation of brain slices. A two-level soft-lithography process is used to fabricate fluid ports with integrated injection and suction holes that are connected to underlying microchannels. Fluorescence imaging, particle tracking velocimetry, and cell staining are used to characterize flows around a fluid port with or without active suction to validate effective localization of injected chemicals. To demonstrate biological applicability of the chamber, we show an induction of cortical spreading depression (CSD) waves in mouse brain slices through controlled focal delivery of potassium chloride solution.

Graphical abstract: Development and characterization of a microfluidic chamber incorporating fluid ports with active suction for localized chemical stimulation of brain slices

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Publication details

The article was received on 04 Mar 2011, accepted on 12 Apr 2011 and first published on 12 May 2011


Article type: Paper
DOI: 10.1039/C1LC20197B
Lab Chip, 2011,11, 2247-2254

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    Development and characterization of a microfluidic chamber incorporating fluid ports with active suction for localized chemical stimulation of brain slices

    Y. T. Tang, J. Kim, H. E. López-Valdés, K. C. Brennan and Y. S. Ju, Lab Chip, 2011, 11, 2247
    DOI: 10.1039/C1LC20197B

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