Jump to main content
Jump to site search

Issue 7, 2011
Previous Article Next Article

Positional dependence of particles in microfludic impedance cytometry

Author affiliations

Abstract

Single cell impedance cytometry is a label-free electrical analysis method that requires minimal sample preparation and has been used to count and discriminate cells on the basis of their impedance properties. This paper shows experimental and numerically simulated impedance signals for test particles (6 μm diameter polystyrene) flowing through a microfluidic channel. The variation of impedance signal with particle position is mapped using numerical simulation and these results match closely with experimental data. We demonstrate that for a nominal 40 μm × 40 μm channel, the impedance signal is independent of position over the majority of the channel area, but shows large experimentally verifiable variation at extreme positions. The parabolic flow profile in the channel ensures that most of the sample flows through the area of uniform signal. At high flow rates inertial focusing is observed; the particles flow in equal numbers through two equilibrium positions reducing the coefficient of variance (CV) in the impedance signals to negligible values.

Graphical abstract: Positional dependence of particles in microfludic impedance cytometry

Back to tab navigation

Article information


Submitted
10 Jan 2011
Accepted
22 Feb 2011
First published
28 Feb 2011

Lab Chip, 2011,11, 1234-1239
Article type
Paper

Positional dependence of particles in microfludic impedance cytometry

D. Spencer and H. Morgan, Lab Chip, 2011, 11, 1234
DOI: 10.1039/C1LC20016J

Social activity

Search articles by author

Spotlight

Advertisements