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Issue 8, 2011
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Open-access and multi-directional electroosmotic flow chip for positioning heterotypic cells

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Abstract

We propose a novel method of cell positioning using electroosmotic flow (EOF) to analyze cell–cell interactions. The EOF chip has an open-to-air configuration, is equipped with four electrodes to induce multi-directional EOF, and allows access of tools for liquid handling and of physical probes for cell measurements. Evaluation of the flow within this chip indicated that it controlled hydrodynamic transport of cells, in terms of both speed and direction. We also evaluated cell viability after EOF application and determined appropriate conditions for cell positioning. Two cells were successively positioned in pocket-like microstructures, one in each micropocket, by controlling the EOF direction. As an experimental demonstration, we observed contact interactions between two individual cells through gap junction channels. The EOF chip should provide ways to elucidate various cell–cell interactions between heterotypic cells.

Graphical abstract: Open-access and multi-directional electroosmotic flow chip for positioning heterotypic cells

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Supplementary files

Article information


Submitted
26 Nov 2010
Accepted
02 Feb 2011
First published
24 Feb 2011

Lab Chip, 2011,11, 1507-1512
Article type
Paper

Open-access and multi-directional electroosmotic flow chip for positioning heterotypic cells

K. Terao, Y. Kitazawa, R. Yokokawa, A. Okonogi and H. Kotera, Lab Chip, 2011, 11, 1507
DOI: 10.1039/C0LC00634C

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