Issue 20, 2010

Development of a multi-layer microfluidic array chip to culture and replate uniform-sized embryoid bodies without manual cell retrieval

Abstract

We have developed a multi-layer, microfluidic array platform containing concave microwells and flat cell culture chambers to culture embryonic stem (ES) cells and regulate uniform-sized embryoid body (EB) formation. The main advantage of this platform was that EBs cultured within the concave microwells of a bottom layer were automatically replated into flat cell culture chambers of a top layer, following inversion of the multi-layer microfluidic array platform. This allowed EB formation and EB replating to be controlled simultaneously inside a single microfluidic device without pipette-based manual cell retrieval, a drawback of previous EB culture methods.

Graphical abstract: Development of a multi-layer microfluidic array chip to culture and replate uniform-sized embryoid bodies without manual cell retrieval

Supplementary files

Article information

Article type
Communication
Submitted
07 May 2010
Accepted
14 Jul 2010
First published
25 Aug 2010

Lab Chip, 2010,10, 2651-2654

Development of a multi-layer microfluidic array chip to culture and replate uniform-sized embryoid bodies without manual cell retrieval

E. Kang, Y. Y. Choi, Y. Jun, B. G. Chung and S. Lee, Lab Chip, 2010, 10, 2651 DOI: 10.1039/C0LC00005A

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