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Issue 20, 2010
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Development of a multi-layer microfluidic array chip to culture and replate uniform-sized embryoid bodies without manual cell retrieval

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Abstract

We have developed a multi-layer, microfluidic array platform containing concave microwells and flat cell culture chambers to culture embryonic stem (ES) cells and regulate uniform-sized embryoid body (EB) formation. The main advantage of this platform was that EBs cultured within the concave microwells of a bottom layer were automatically replated into flat cell culture chambers of a top layer, following inversion of the multi-layer microfluidic array platform. This allowed EB formation and EB replating to be controlled simultaneously inside a single microfluidic device without pipette-based manual cell retrieval, a drawback of previous EB culture methods.

Graphical abstract: Development of a multi-layer microfluidic array chip to culture and replate uniform-sized embryoid bodies without manual cell retrieval

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Article information


Submitted
07 May 2010
Accepted
14 Jul 2010
First published
25 Aug 2010

Lab Chip, 2010,10, 2651-2654
Article type
Communication

Development of a multi-layer microfluidic array chip to culture and replate uniform-sized embryoid bodies without manual cell retrieval

E. Kang, Y. Y. Choi, Y. Jun, B. G. Chung and S. Lee, Lab Chip, 2010, 10, 2651
DOI: 10.1039/C0LC00005A

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