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Issue 19, 2010

Micropillar array chips toward new immunodiagnosis

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Abstract

In this paper, we demonstrate the possibility to use a micropillar array to perform molecular immunodiagnosis. A polydimethylsiloxane (PDMS) microdevice consisting of a rectangular array of micropillars (45 μm in height, 100 × 100 μm square cross section) was used to replace microchannels or gels (polyacrylamide or agarose) to perform electrokinetic separation. This microarray was used to mimic highly diluted gel and to maintain electrolyte within the pillar zone by capillary effect. The electrolyte composition (glycerol and agarose content) was investigated in order to improve protein separation by isoelectric focusing (IEF). The influence of glycerol on focusing time and on the different evaporative contributions was further evaluated. In order to perform an immunodiagnostic of milk allergy, different surface treatments were optimized to prevent milk allergen adsorption on PDMS surface. Poly(dimethylacrylamide)-co-allyl glycidyl ether (PDMA-AGE) as well as gelatin led to a satisfactory signal to noise ratio. Finally the possibility to perform protein mixture separation using this micropillar array chip followed by immunoblotting was demonstrated by using the serum from an allergic individual, confirming the great potential of this analytical platform in the field of immunodiagnosis.

Graphical abstract: Micropillar array chips toward new immunodiagnosis

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Article information


Submitted
12 Apr 2010
Accepted
14 Jun 2010
First published
16 Aug 2010

Lab Chip, 2010,10, 2597-2604
Article type
Paper

Micropillar array chips toward new immunodiagnosis

H. Li, V. Dauriac, V. Thibert, H. Senechal, G. Peltre, X. Zhang and S. Descroix, Lab Chip, 2010, 10, 2597 DOI: 10.1039/C005034B

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