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Issue 8, 2010
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Electrokinetic label-free screening chip: a marriage of multiplexing and high throughput analysis using surface plasmon resonance imaging

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Abstract

We present an electrokinetic label-free biomolecular screening chip (Glass/PDMS) to screen up to 10 samples simultaneously using surface plasmon resonance imaging (iSPR). This approach reduces the duration of an experiment when compared to conventional experimental methods. This new device offers a high degree of parallelization not only for analyte samples, but also for multiplex analyte interactions where up to 90 ligands are immobilized on the sensing surface. The proof of concept has been demonstrated with well-known biomolecular interactant pairs. The new chip can be used for high throughput screening applications and kinetics parameter extraction, simultaneously, of interactant–protein complex formation.

Graphical abstract: Electrokinetic label-free screening chip: a marriage of multiplexing and high throughput analysis using surface plasmon resonance imaging

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Submitted
12 Jan 2010
Accepted
10 Feb 2010
First published
09 Mar 2010

Lab Chip, 2010,10, 986-990
Article type
Communication

Electrokinetic label-free screening chip: a marriage of multiplexing and high throughput analysis using surface plasmon resonance imaging

G. Krishnamoorthy, E. T. Carlen, J. G. Bomer, D. Wijnperlé, H. L. deBoer, A. van den Berg and R. B. M. Schasfoort, Lab Chip, 2010, 10, 986
DOI: 10.1039/C000705F

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