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Issue 4, 2010
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Selective isolation of live/dead cells using contactless dielectrophoresis (cDEP)

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Abstract

Contactless dielectrophoresis (cDEP) is a recently developed method of cell manipulation in which the electrodes are physically isolated from the sample. Here we present two microfluidic devices capable of selectively isolating live human leukemia cells from dead cells utilizing their electrical signatures. The effect of different voltages and frequencies on the gradient of the electric field and device performance was investigated numerically and validated experimentally. With these prototype devices we were able to achieve greater than 95% removal efficiency at 0.2–0.5 mm s−1 with 100% selectivity between live and dead cells. In conjunction with enrichment, cDEP could be integrated with other technologies to yield fully automated lab-on-a-chip systems capable of sensing, sorting, and identifying rare cells.

Graphical abstract: Selective isolation of live/dead cells using contactless dielectrophoresis (cDEP)

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Supplementary files

Article information


Submitted
02 Oct 2009
Accepted
14 Dec 2009
First published
19 Jan 2010

Lab Chip, 2010,10, 438-445
Article type
Paper

Selective isolation of live/dead cells using contactless dielectrophoresis (cDEP)

H. Shafiee, M. B. Sano, E. A. Henslee, J. L. Caldwell and R. V. Davalos, Lab Chip, 2010, 10, 438
DOI: 10.1039/B920590J

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