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Issue 3, 2010
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Microfluidic devices for cell based high throughput screening

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Abstract

Cell based screening assays are increasingly used in drug discovery due to the physiological significance of the results and high content information obtained from them. Miniaturization of this format, currently carried out in microwell plates, is at its limit due to increased unnatural interaction of cells with walls inside micro-wells. In order to overcome this limitation, we present a new format for dynamically controlled, precise, spatial and temporal dosing of a continuous cell culture layer, using microfluidics. The device consists of a micropatterned nanoporous membrane layer that allows specific spatial locations in the continuous gel layer above, to be chemically addressed by external electric field through a microfluidic network below it. We demonstrate that the control of electric field across the nanoporous membrane leads to extremely precise dosing (∼ 50 µg accuracy). Spot sizes of 200 µm to 6 mm in diameter and inter-spot distances of 0.4–10 mm have been obtained. Microarray spot densities of 156 spots/cm2 were obtained, which is five times higher than the densities used in current cell based assays. The capability of this method in handling small molecules, proteins and drugs is also demonstrated. This format of spatial dosing of continuous cell culture will enable further miniaturization of cell based assays and aid in high-throughput high-content screening.

Graphical abstract: Microfluidic devices for cell based high throughput screening

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Publication details

The article was received on 04 Sep 2009, accepted on 04 Nov 2009 and first published on 27 Nov 2009


Article type: Paper
DOI: 10.1039/B918291H
Lab Chip, 2010,10, 341-348

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    Microfluidic devices for cell based high throughput screening

    S. Upadhyaya and P. R. Selvaganapathy, Lab Chip, 2010, 10, 341
    DOI: 10.1039/B918291H

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