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Issue 23, 2009
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Hands-free sample preparation platform for nucleic acid analysis

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Abstract

A Lab-On-Chip system with an instrument is presented which is capable of performing total sample preparation and automated extraction of nucleic acid from human cell samples fixed in a methanol based solution. The target application is extraction of mRNA from cervical liquid based cytology specimens for detection of transformed HPV-infections. The device accepts 3 ml of sample and performs the extraction in a disposable polymer chip of credit card size. All necessary reagents for cell lysis, washing, and elution are stored on-chip and the extraction is performed in two filter stages; one for cell pre-concentration and the other for nucleic acid capture. Tests performed using cancer cell lines and cervical liquid based cytology specimens confirm the extraction of HPV-mRNA by the system.

Graphical abstract: Hands-free sample preparation platform for nucleic acid analysis

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Article information


Submitted
27 May 2009
Accepted
14 Aug 2009
First published
30 Sep 2009

Lab Chip, 2009,9, 3399-3405
Article type
Paper

Hands-free sample preparation platform for nucleic acid analysis

T. Baier, T. E. Hansen-Hagge, R. Gransee, A. Crombé, S. Schmahl, C. Paulus, K. S. Drese, H. Keegan, C. Martin, J. J. O'Leary, L. Furuberg, L. Solli, P. Grønn, I. M. Falang, A. Karlgård, A. Gulliksen and F. Karlsen, Lab Chip, 2009, 9, 3399
DOI: 10.1039/B910421F

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