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Issue 21, 2009
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Lab-on-Chip for fast 3D particle tracking in living cells

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Abstract

We present a novel Lab-on-Chip technology for 3D particle tracking in living cells based on V-shaped micro-mirrors, which are used to observe fluorescent specimens from multiple vantage points, providing simultaneous stereo-images that can be recombined for 3D reconstruction. Our technology can be readily used with standard fluorescence microscopes, and we apply it to study chromatin dynamics using yeast strains with one or two GFP-tagged gene loci. Using an Andor EMCCD camera, loci are followed in 3D with inter-frame intervals of up to 10 ms and with an error of 27 nm per axis, yielding quantitative information on their dynamics with exquisite temporal spatial resolution.

Graphical abstract: Lab-on-Chip for fast 3D particle tracking in living cells

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Article information


Submitted
06 May 2009
Accepted
13 Aug 2009
First published
02 Sep 2009

Lab Chip, 2009,9, 3054-3058
Article type
Paper

Lab-on-Chip for fast 3D particle tracking in living cells

H. Hajjoul, S. Kocanova, I. Lassadi, K. Bystricky and A. Bancaud, Lab Chip, 2009, 9, 3054
DOI: 10.1039/B909016A

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