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Issue 13, 2009
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Multi-wavelength microflow cytometer using groove-generated sheath flow

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Abstract

A microflow cytometer was developed that ensheathed the sample (core) fluid on all sides and interrogated each particle in the sample stream at four different wavelengths. Sheathing was achieved by first sandwiching the core fluid with the sheath fluid laterally via fluid focusing. Chevron-shaped groove features fabricated in the top and bottom of the channel directed sheath fluid from the sides to the top and bottom of the channel, completely surrounding the sample stream. Optical fibers inserted into guide channels provided excitation light from diode lasers at 532 and 635 nm and collected the emission wavelengths. Two emission collection fibers were connected to PMTs through a multimode fiber splitter and optical filters for detection at 635 nm (scatter), 665 nm and 700 nm (microsphere identification) and 565 nm (phycoerythrin tracer). The cytometer was capable of discriminating microspheres with different amounts of the fluorophores used for coding and detecting the presence of a phycoerythrin antibody complex on the surface of the microspheres. Assays for Escherichia coli were compared with a commercial Luminex flow cytometer.

Graphical abstract: Multi-wavelength microflow cytometer using groove-generated sheath flow

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Article information


Submitted
15 Dec 2008
Accepted
16 Mar 2009
First published
31 Mar 2009

Lab Chip, 2009,9, 1942-1950
Article type
Paper

Multi-wavelength microflow cytometer using groove-generated sheath flow

J. P. Golden, J. S. Kim, J. S. Erickson, L. R. Hilliard, P. B. Howell, G. P. Anderson, M. Nasir and F. S. Ligler, Lab Chip, 2009, 9, 1942
DOI: 10.1039/B822442K

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