Jump to main content
Jump to site search

Issue 10, 2009
Previous Article Next Article

Effects of carbon dioxide on peak mode isotachophoresis: Simultaneous preconcentration and separation

Author affiliations

Abstract

We present a method that achieves simultaneous preconcentration and separation of analytes using peak-mode isotachophoresis with a single step injection in simple, off-the-shelf microchannels or capillaries. We leverage ions resulting from dissolved atmospheric carbon dioxide to weakly disrupt isotachophoretic preconcentration and induce separation of analyte species. We experimentally study the region between the leading and trailing electrolytes, and individually identify the carbonate and carbamate zones that result from the hydration and carbamation reaction of dissolved atmospheric carbon dioxide, respectively. The width of these zones and the gradient regions between them grow with time and create an electric field gradient that causes analytes to separate. Using this assay, we achieve focusing and separation of a 25 bp DNA ladder in a straight, 34 µm wide microchannel in a single loading step. As a demonstration of the fractionation capabilities of the assay, we show simultaneous preconcentration and separation of a DNA ladder from two proteins, GFP and allophycocyanin.

Graphical abstract: Effects of carbon dioxide on peak mode isotachophoresis: Simultaneous preconcentration and separation

Back to tab navigation

Supplementary files

Publication details

The article was received on 04 Sep 2008, accepted on 10 Feb 2009 and first published on 12 Mar 2009


Article type: Paper
DOI: 10.1039/B815460K
Lab Chip, 2009,9, 1377-1384

  •   Request permissions

    Effects of carbon dioxide on peak mode isotachophoresis: Simultaneous preconcentration and separation

    T. K. Khurana and J. G. Santiago, Lab Chip, 2009, 9, 1377
    DOI: 10.1039/B815460K

Search articles by author

Spotlight

Advertisements