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Issue 3, 2009
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Controlled cocultures of HeLa cells and human umbilical vein endothelial cells on detachable substrates

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Abstract

We investigated the interactions between HeLa cells and human umbilical vein endothelial cells (HUVECs) by monitoring their movements in a controllable coculture system. Two complementary, detachable, cell-substrates, one of polystyrene (PS) and the other of poly(dimethylsiloxane) (PDMS), were fabricated by replica molding. Coculturing was started by mechanically assembling two complementary substrates. One substrate was covered with a confluent layer of HeLa cells and its complement covered with confluent HUVECs. Using this coculture system as a tumor/endothelium model, we found that the HeLa cells migrated towards the HUVECs, while, simultaneously, the HUVECs retreated and that both types of cells migrated approximately twice as rapidly (two hundred microns per twenty-four hours) as they did alone. Additionally, when direct contact between the two cell types was prevented, the HUVECs initially migrated towards the HeLa cells and then retreated. The characteristics of the cell movements, i.e. direction and speed, probably are consequences of cell–cell signaling, with such signals possibly important during tumor cell intra- and extravasation.

Graphical abstract: Controlled cocultures of HeLa cells and human umbilical vein endothelial cells on detachable substrates

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Supplementary files

Article information


Submitted
21 Jul 2008
Accepted
06 Oct 2008
First published
06 Nov 2008

Lab Chip, 2009,9, 427-432
Article type
Paper

Controlled cocultures of HeLa cells and human umbilical vein endothelial cells on detachable substrates

H. Kaji, T. Yokoi, T. Kawashima and M. Nishizawa, Lab Chip, 2009, 9, 427
DOI: 10.1039/B812510D

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