Issue 4, 2009

Protein self-interaction chromatography on a microchip

Abstract

This paper presents the development of a novel miniaturized experimental procedure for the measurement of proteinprotein interactions through Self-Interaction Chromatography (SIC) on a microchip, without the use of chromatographic resins. SIC was recently demonstrated to be a relatively easy method to obtain quantitative thermodynamic information about proteinprotein interactions, like the osmotic second virial coefficient B22, which relates to protein phase behavior including protein crystallization. This successful miniaturization to microchip level of a measurement device for protein self-interaction data is a first key step to a complete microfluidic screening platform for the rational design of protein crystallizations, using substantially less expensive protein and experimentation time.

Graphical abstract: Protein self-interaction chromatography on a microchip

Article information

Article type
Paper
Submitted
03 Jul 2008
Accepted
30 Oct 2008
First published
21 Nov 2008

Lab Chip, 2009,9, 600-605

Protein self-interaction chromatography on a microchip

K. Deshpande, T. Ahamed, L. A. M. van der Wielen, J. H. T. Horst, P. J. Jansens and M. Ottens, Lab Chip, 2009, 9, 600 DOI: 10.1039/B810741F

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