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Issue 8, 2008
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Concentration control for protein crystallizationvia a continuously-fed crystallization chamber

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Abstract

A continuously-fed crystallization chamber that allows for kinetic path control through the crystallization phase diagram (from labile/nucleation to metastable/growth) was fabricated and used to crystallize lysozyme. A lumped kinetic model was developed, and parameters for heterogeneous nucleation kinetics were determined. Heterogeneous nucleation was found to have faster nucleation kinetics and slower growth kinetics than homogeneous nucleation, as expected. The major contributions of the new device are (1) to allow better control of the chemical environment for studies of crystal nucleation and growth, and (2) to allow lumped-model analysis of those studies to extract kinetic parameters.

Graphical abstract: Concentration control for protein crystallizationvia a continuously-fed crystallization chamber

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Article information


Submitted
31 Jan 2008
Accepted
09 May 2008
First published
19 Jun 2008

Lab Chip, 2008,8, 1398-1401
Article type
Technical Note

Concentration control for protein crystallizationvia a continuously-fed crystallization chamber

M. Sugiyama, S. Sengupta, P. Todd and V. H. Barocas, Lab Chip, 2008, 8, 1398
DOI: 10.1039/B801686K

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