Issue 1, 2008

Microfabricated valveless devices for thermal bioreactions based on diffusion-limited evaporation

Abstract

Microfluidic devices that reduce evaporative loss during thermal bioreactions such as PCR without microvalves have been developed by relying on the principle of diffusion-limited evaporation. Both theoretical and experimental results demonstrate that the sample evaporative loss can be reduced by more than 20 times using long narrow diffusion channels on both sides of the reaction region. In order to further suppress the evaporation, the driving force for liquid evaporation is reduced by two additional techniques: decreasing the interfacial temperature using thermal isolation and reducing the vapor concentration gradient by replenishing water vapor in the diffusion channels. Both thermal isolation and vapor replenishment techniques can limit the sample evaporative loss to approximately 1% of the reaction content.

Graphical abstract: Microfabricated valveless devices for thermal bioreactions based on diffusion-limited evaporation

Supplementary files

Article information

Article type
Paper
Submitted
01 Aug 2007
Accepted
09 Oct 2007
First published
31 Oct 2007

Lab Chip, 2008,8, 88-97

Microfabricated valveless devices for thermal bioreactions based on diffusion-limited evaporation

F. Wang, M. Yang and M. A. Burns, Lab Chip, 2008, 8, 88 DOI: 10.1039/B711770A

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