Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance work on Wednesday 21st October 2020 from 07:00 AM to 07:00 PM (BST).

During this time our website performance may be temporarily affected. We apologise for any inconvenience this might cause and thank you for your patience.


Issue 6, 2007
Previous Article Next Article

A practical guide to microfluidic perfusion culture of adherent mammalian cells

Author affiliations

Abstract

Culturing cells at microscales allows control over microenvironmental cues, such as cell–cell and cell–matrix interactions; the potential to scale experiments; the use of small culture volumes; and the ability to integrate with microsystem technologies for on-chip experimentation. Microfluidic perfusion culture in particular allows controlled delivery and removal of soluble biochemical molecules in the extracellular microenvironment, and controlled application of mechanical forces exerted via fluid flow. There are many challenges to designing and operating a robust microfluidic perfusion culture system for routine culture of adherent mammalian cells. The current literature on microfluidic perfusion culture treats microfluidic design, device fabrication, cell culture, and micro-assays independently. Here we systematically present and discuss important design considerations in the context of the entire microfluidic perfusion culture system. These design considerations include the choice of materials, culture configurations, microfluidic network fabrication and micro-assays. We also present technical issues such as sterilization; seeding cells in both 2D and 3D configurations; and operating the system under optimized mass transport and shear stress conditions, free of air-bubbles. The integrative and systematic treatment of the microfluidic system design and fabrication, cell culture, and micro-assays provides novices with an effective starting point to build and operate a robust microfludic perfusion culture system for various applications.

Graphical abstract: A practical guide to microfluidic perfusion culture of adherent mammalian cells

Back to tab navigation

Supplementary files

Article information


Submitted
28 Mar 2007
Accepted
16 Apr 2007
First published
11 May 2007

Lab Chip, 2007,7, 681-694
Article type
Tutorial Review

A practical guide to microfluidic perfusion culture of adherent mammalian cells

L. Kim, Y. Toh, J. Voldman and H. Yu, Lab Chip, 2007, 7, 681
DOI: 10.1039/B704602B

Social activity

Search articles by author

Spotlight

Advertisements