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Issue 8, 2006
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Monolithically integrated dye-doped PDMS long-pass filters for disposable on-chip fluorescence detection

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Abstract

We report the fabrication of high quality monolithically integrated optical long-pass filters, for use in disposable diagnostic microchips. The filters were prepared by incorporating dye molecules directly into the microfluidic chip substrate, thereby providing a fully integrated solution that removes the usual need for discrete optical filters. In brief, lysochrome dyes were added to a poly(dimethylsiloxane) (PDMS) monomer prior to moulding of the microchip from a structured SU-8 master. Optimum results were obtained using 1 mm layers of PDMS doped with 1200 µg mL−1 Sudan II, which resulted in less than 0.01% transmittance below 500 nm (OD 4), >80% above 570 nm, and negligible autofluorescence. These spectral characteristics compare favourably with commercially available Schott-glass long-pass filters, indicating that high quality optical filters can be straightforwardly integrated into the form of PDMS microfluidic chips. The filters were found to be robust in use, showing only slight degradation after extended illumination and negligible dye leaching after prolonged exposure to aqueous solutions. The provision of low cost high quality integrated filters represents a key step towards the development of high-sensitivity disposable microfluidic devices for point-of-care diagnostics.

Graphical abstract: Monolithically integrated dye-doped PDMS long-pass filters for disposable on-chip fluorescence detection

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Article information


Submitted
10 Mar 2006
Accepted
17 May 2006
First published
09 Jun 2006

Lab Chip, 2006,6, 981-987
Article type
Paper

Monolithically integrated dye-doped PDMS long-pass filters for disposable on-chip fluorescence detection

O. Hofmann, X. Wang, A. Cornwell, S. Beecher, A. Raja, D. D. C. Bradley, A. J. deMello and J. C. deMello, Lab Chip, 2006, 6, 981
DOI: 10.1039/B603678C

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