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Issue 6, 2005
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Confocal restricted-height imaging of suspension cells (CRISC) in a PDMS microdevice during apoptosis

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Abstract

We have monitored and imaged cell death induced in human leukemic U937 cells over time using three-color confocal imaging. Three different apoptotic inducers, anti-Fas, TNF-α and Etoposide were used. Individual cascaded events such as loss of mitochondrial transmembrane potential, exposure of phosphatidyl-serine, membrane blebbing and permeabilization of the cell membrane have been observed in real time with different individual cells. From the results, an interesting heterogeneicity in the apoptotic phenotype has been observed. The CRISC method is easy to use and provides biologist with a powerful additional tool to study in real-time processes of several hours of duration such as apoptosis. We predict that the period of cell viability obtained after protein coating of the PDMS devices (>80 h) will also allow monitoring of other biological processes of longer duration or long onset time, such as mitosis, phagocytosis and differentiation.

Graphical abstract: Confocal restricted-height imaging of suspension cells (CRISC) in a PDMS microdevice during apoptosis

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Publication details

The article was received on 14 Mar 2005, accepted on 14 Apr 2005 and first published on 09 May 2005


Article type: Paper
DOI: 10.1039/B503770K
Lab Chip, 2005,5, 628-633

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    Confocal restricted-height imaging of suspension cells (CRISC) in a PDMS microdevice during apoptosis

    C. Muñoz-Pinedo, D. R. Green and A. van den Berg, Lab Chip, 2005, 5, 628
    DOI: 10.1039/B503770K

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