Issue 12, 2005

On chip single-cell separation and immobilization using optical tweezers and thermosensitive hydrogel

Abstract

A novel approach appropriate for rapid separation and immobilization of a single cell by concomitantly utilizing laser manipulation and locally thermosensitive hydrogelation is proposed in this paper. We employed a single laser beam as optical tweezers for separating a target cell and locating it adjacent to a fabricated, transparent micro heater. Simultaneously, the target cell is immobilized or partially entrapped by heating the thermosensitive hydrogel with the micro heater. The state of the thermosensitive hydrogel can be switched from sol to gel and gel to sol by controlling the temperature through heating and cooling by the micro heater. After other unwanted cells are removed by the high-speed cleaning flow in the microchannel, the entrapped cell is successfully isolated. It is possible to collect the immobilized target cell for analysis or culture by switching off the micro heater and releasing the cell from the entrapment. We demonstrated that the proposed approach is feasible for rapid manipulation, immobilization, cleaning, isolation and extraction of a single cell. The experimental results are shown here.

Graphical abstract: On chip single-cell separation and immobilization using optical tweezers and thermosensitive hydrogel

Article information

Article type
Paper
Submitted
18 Feb 2005
Accepted
27 Sep 2005
First published
17 Oct 2005

Lab Chip, 2005,5, 1399-1403

On chip single-cell separation and immobilization using optical tweezers and thermosensitive hydrogel

F. Arai, C. Ng, H. Maruyama, A. Ichikawa, H. El-Shimy and T. Fukuda, Lab Chip, 2005, 5, 1399 DOI: 10.1039/B502546J

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