SCHEDULED MAINTENANCE
An artificial gene was constructed combining the T7 promoter and terminator with the EGFP-gene from the plasmid pEGFP. The functionality of the construct was shown by in vitro translation. The gene-construct was immobilised on a planar glass surface. The transcription was performed on the immobilised gene and mRNA was determined by RT-PCR. Multiple use of the immobilised gene was demonstrated.
J. Steffen, M. von Nickisch-Rosenegk and F. F. Bier, Lab Chip, 2005, 5, 665 DOI: 10.1039/B501091H
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