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Issue 4, 2005
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Effects of the cell geometry and operating parameters on the performance of an external contactless conductivity detector for microchip electrophoresis

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Abstract

Quantitative data on the effect of the electrode geometry on the signal strength and the signal-to-noise ratio is given. The measurements are affected by the unavoidable presence of stray capacitance. Best results are achieved for short and narrow electrodes arranged in an antiparallel configuration and separated by a minimal gap, which determines the dimensions of the actual detection volume. Limits of detection between 150 and 250 µg l−1 and separation efficiencies from 13 000 to 17 000 theoretical plates were achieved for six inorganic cations (NH4+, K+, Ca2+, Na+, Mg2+and Li+) with electrodes of 1 mm width and a detection gap of 0.5 mm (separation channel length: 7.5 cm) when operating the detector at 20 Vpp and 500 kHz. The analyses of all major inorganic cations in tap and rain water samples were demonstrated for the first time in microchip electrophoresis with contactless conductivity detection.

Graphical abstract: Effects of the cell geometry and operating parameters on the performance of an external contactless conductivity detector for microchip electrophoresis

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Article information


Submitted
15 Dec 2004
Accepted
23 Feb 2005
First published
04 Mar 2005

Lab Chip, 2005,5, 407-415
Article type
Paper

Effects of the cell geometry and operating parameters on the performance of an external contactless conductivity detector for microchip electrophoresis

P. Kubáň and P. C. Hauser, Lab Chip, 2005, 5, 407
DOI: 10.1039/B418845D

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