Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.


Issue 3, 2005
Previous Article Next Article

Thin chip microsprayer system coupled to quadrupole time-of-flight mass spectrometer for glycoconjugate analysis

Author affiliations

Abstract

A thin chip polymer-based microsprayer has been coupled to a hybrid quadrupole time-of-flight mass spectrometer (QTOF MS) and introduced in carbohydrate research. The feasibility of the approach is demonstrated for mapping, sequencing and structural elucidation of glycoconjugates originating from human body fluids and tissues such as a glycopeptide mixture from normal human urine and an isolated and purified GT1 ganglioside fraction from normal adult human brain. The optimization procedure required by each glycoconjugate category is described and the advantages of the system in terms of flexibility and adaptability to QTOF MS, stability of the ESI MS signal, carbohydrate ionization and sequencing, sensitivity, speed of analysis and sample consumption are discussed.

Graphical abstract: Thin chip microsprayer system coupled to quadrupole time-of-flight mass spectrometer for glycoconjugate analysis

Back to tab navigation

Article information


Submitted
01 Sep 2004
Accepted
09 Nov 2004
First published
22 Dec 2004

Lab Chip, 2005,5, 298-307
Article type
Paper

Thin chip microsprayer system coupled to quadrupole time-of-flight mass spectrometer for glycoconjugate analysis

A. D. Zamfir, N. Lion, Ž. Vukelić, L. Bindila, J. Rossier, H. H. Girault and J. Peter-Katalinić, Lab Chip, 2005, 5, 298
DOI: 10.1039/B413282C

Social activity

Search articles by author

Spotlight

Advertisements