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Issue 1, 2005
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Integrated microelectrode array and microfluidics for temperature clamp of sensory neurons in culture

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Abstract

A device for cell culture is presented that combines MEMS technology and liquid-phase photolithography to create a microfluidic chip that influences and records electrical cellular activity. A photopolymer channel network is formed on top of a multichannel microelectrode array. Preliminary results indicated successful local thermal control within microfluidic channels and control of lamina position over the electrode array. To demonstrate the biological application of such a device, adult dissociated dorsal root ganglion neurons with a subpopulation of thermally-sensitive cells are attached onto the electrode array. Using laminar flow, dynamic control of local temperature of the neural cells was achieved while maintaining a constant chemical culture medium. Recording the expected altered cellular activity confirms the success of the integrated device.

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Article information


Submitted
26 May 2004
Accepted
17 Jun 2004
First published
14 Sep 2004

Lab Chip, 2005,5, 97-101
Article type
Paper

Integrated microelectrode array and microfluidics for temperature clamp of sensory neurons in culture

T. M. Pearce, J. A. Wilson, S. G. Oakes, S. Chiu and J. C. Williams, Lab Chip, 2005, 5, 97 DOI: 10.1039/B407871C

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