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Issue 1, 2005
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Integrated microelectrode array and microfluidics for temperature clamp of sensory neurons in culture

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Abstract

A device for cell culture is presented that combines MEMS technology and liquid-phase photolithography to create a microfluidic chip that influences and records electrical cellular activity. A photopolymer channel network is formed on top of a multichannel microelectrode array. Preliminary results indicated successful local thermal control within microfluidic channels and control of lamina position over the electrode array. To demonstrate the biological application of such a device, adult dissociated dorsal root ganglion neurons with a subpopulation of thermally-sensitive cells are attached onto the electrode array. Using laminar flow, dynamic control of local temperature of the neural cells was achieved while maintaining a constant chemical culture medium. Recording the expected altered cellular activity confirms the success of the integrated device.

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Publication details

The article was received on 26 May 2004, accepted on 17 Jun 2004 and first published on 14 Sep 2004


Article type: Paper
DOI: 10.1039/B407871C
Lab Chip, 2005,5, 97-101

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    Integrated microelectrode array and microfluidics for temperature clamp of sensory neurons in culture

    T. M. Pearce, J. A. Wilson, S. G. Oakes, S. Chiu and J. C. Williams, Lab Chip, 2005, 5, 97
    DOI: 10.1039/B407871C

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