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Issue 4, 2004
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Combinatorial mixing of microfluidic streams

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We have devised a microfluidic mixer design that produces all the mixture combinations of a given number of dilutions of the input compounds. As proof of the concept, we present a device that generates four titrations of two dye solutions, blue and yellow, and combinatorially mixes the blue titrations with the yellow titrations to deliver the sixteen mixture combinations in separate outlet microchannels. Our device features four different flow levels made by stacking nine laser-cut Mylar laminates. The fluidic network has a symmetric design that guarantees that the flow rates are the same at all the outlets, with deviations attributable to imperfections in the fabrication, assembly, or perfusion processes. Design rules for scaling up the number of compounds and/or dilutions are presented. The mixing scheme has broad applicability in high-throughput combinatorial testing applications such as drug screening, cell-based biochemical assays, lab-on-a-chip devices, and biosensors.

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Publication details

The article was received on 19 Nov 2003, accepted on 22 Mar 2004 and first published on 23 Apr 2004

Article type: Paper
DOI: 10.1039/B314962E
Citation: Lab Chip, 2004,4, 342-350

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    Combinatorial mixing of microfluidic streams

    C. Neils, Z. Tyree, B. Finlayson and A. Folch, Lab Chip, 2004, 4, 342
    DOI: 10.1039/B314962E

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