Issue 2, 2004

Thin-film polymer light emitting diodes as integrated excitation sources for microscale capillary electrophoresis

Abstract

We report the use of a thin-film polymer light emitting diode as an integrated excitation source for microfabricated capillary electrophoresis. The polyfluorene-based diode has a peak emission wavelength of 488 nm, an active area of 40 µm × 1000 µm and a thickness of ∼2 mm. The simple layer-by-layer deposition procedures used to fabricate the polymer component allow facile integration with planar chip-based systems. To demonstrate the efficacy of the approach, the polyfluorene diode is used as an excitation source for the detection of fluorescent dyes separated on-chip by electrophoresis. Using a conventional confocal detection system the integrated pLED is successfully used to detect fluorescein and 5-carboxyfluorescein at concentrations as low as 10−6 M with a mass detection limit of 50 femtomoles. The drive voltages required to generate sufficient emission from the polymer diode device are as low as 3.7 V.

Article information

Article type
Paper
Submitted
24 Oct 2003
Accepted
24 Dec 2003
First published
04 Feb 2004

Lab Chip, 2004,4, 136-140

Thin-film polymer light emitting diodes as integrated excitation sources for microscale capillary electrophoresis

J. B. Edel, N. P. Beard, O. Hofmann, J. C. deMello, D. D. C. Bradley and A. J. deMello, Lab Chip, 2004, 4, 136 DOI: 10.1039/B313503A

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