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Issue 3, 2004
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Electrophoretic manipulation of single DNA molecules in nanofabricated capillaries

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Abstract

We demonstrate the use of nanofabricated capillaries, integrated as part of a microfluidic structure, to study the electrophoretic behaviour of single, fluorescently-labelled, molecules of DNA as a function of capillary size. The nanocapillaries, fabricated using a focused ion beam, have cross-sections down to 150 × 180 nm. Control of single-molecule direction and velocity was achieved using voltage manipulation. DNA mobility was found to increase with decreasing cross-section, which we interpret in terms of reduced electro-osmotic counter-flow. Such nanofabricated capillaries as part of larger fluidic structures have great potential for biotechnology, particularly single molecule manipulation and analysis.

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Publication details

The article was received on 08 Oct 2003, accepted on 09 Feb 2004 and first published on 12 Mar 2004


Article type: Paper
DOI: 10.1039/B312592K
Citation: Lab Chip, 2004,4, 225-229

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    Electrophoretic manipulation of single DNA molecules in nanofabricated capillaries

    L. C. Campbell, M. J. Wilkinson, A. Manz, P. Camilleri and C. J. Humphreys, Lab Chip, 2004, 4, 225
    DOI: 10.1039/B312592K

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