Jump to main content
Jump to site search

Issue 1, 2003
Previous Article Next Article

A novel microfluidic concept for bioanalysis using freely moving beads trapped in recirculating flows

Author affiliations

Abstract

There are only a few examples in which beads are employed for heterogeneous assays on microfluidic devices, because of the difficulties associated with packing and handling these in etched microstructures. This contribution describes a microfluidic device that allows the capture, preconcentration, and controlled manipulation of small beads (<6 μm) in etched microchannels using fluid flows only. The chips feature planar diverging and converging channel elements connected by a narrow microchannel. Creation of bi-directional liquid movement by opposing electro-osmotic and pressure-driven flows can lead to the generation of controlled recirculating flow at these elements. Small polymer beads can actually be captured in the controlled rotating flow patterns. The clusters of freely moving beads that result can be perfused sequentially with different solutions. A preliminary binding curve was determined for the reaction of streptavidin-coated beads and fluorescein-labelled biotin, demonstrating the potential of this bead-handling approach for bioanalysis.

Back to tab navigation

Article information


Submitted
29 Nov 2002
Accepted
21 Jan 2003
First published
10 Feb 2003

Lab Chip, 2003,3, 34-39
Article type
Paper

A novel microfluidic concept for bioanalysis using freely moving beads trapped in recirculating flows

G. Lettieri, A. Dodge, G. Boer, N. F. de Rooij and E. Verpoorte, Lab Chip, 2003, 3, 34
DOI: 10.1039/B211869F

Social activity

Search articles by author

Spotlight

Advertisements