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Issue 38, 2011
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One-pot synthesis of N,N-bis[2-methylbutyl] imidazolium hexafluorophosphate–TiO2 nanocomposites and application for protein isolation

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Abstract

A novel one-pot procedure for the synthesis of ionic liquid N,N-bis[2-methylbutyl] imidazolium hexafluorophosphate (PPimPF6/TiO2) nanocomposites is reported for the first time. During the in situ formation of TiO2 nanoparticles via hydrolysis of tetrabutyl titanate (TBOT), the ionic liquid PPimPF6 is simultaneously incorporated into the TiO2 nanostructure via H-bonding. The PPimPF6/TiO2 nanocomposites are characterized by means of TEM, FT-IR and XRD, showing that they possess the structure of anatase and exhibit selective adsorption for neutral proteins, hemoglobin (Hb) in this particular case, under controlled experimental conditions. At pH 8, an adsorption efficiency of 88.3% for 150 μg mL−1 Hb is achieved by 2.0 mg of PPimPF6/TiO2 nanocomposites, while virtually no adsorption is observed for the basic protein cytochrome c (cyt-c) and the acidic protein bovine serum albumin (BSA). An adsorption capacity of 122.3 μg mg−1 is obtained for Hb by calculation on the amount of the incorporated ionic liquid, corresponding to a 5-fold improvement with respect to that achieved by pure PPimPF6. The retained Hb on the PPimPF6/TiO2 nanocomposites could be readily collected by stripping with 0.5% SDS, giving rise to a recovery of ca. 73%. The circular dichroism (CD) spectra indicate no conformational change of Hb after the adsorption/elution process. The selective isolation of Hb from human whole blood is achieved by the nanocomposites.

Graphical abstract: One-pot synthesis of N,N-bis[2-methylbutyl] imidazolium hexafluorophosphate–TiO2 nanocomposites and application for protein isolation

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Article information


Submitted
02 May 2011
Accepted
14 Jul 2011
First published
18 Aug 2011

J. Mater. Chem., 2011,21, 14857-14863
Article type
Paper

One-pot synthesis of N,N-bis[2-methylbutyl] imidazolium hexafluorophosphateTiO2 nanocomposites and application for protein isolation

H. Meng, X. Chen and J. Wang, J. Mater. Chem., 2011, 21, 14857
DOI: 10.1039/C1JM11918D

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