Issue 29, 2011
From the journal:

Journal of Materials Chemistry

Fabrication, microstructure, and BMP-2 delivery of novel biodegradable and biocompatible silicate–collagen hybrid fibril sheets

Song Chen,a   Akiyoshi Osaka,b   Toshiyuki Ikoma,c   Hiromi Morita,a   Jie Li,d   Masaki Takeguchie  and  Nobutaka Hanagata*a  

* Corresponding authors

a Biomaterials Center, National Institute for Materials Science, 1-2-1 Sengen, Tsukuba, Ibaraki, Japan
E-mail: HANAGATA.Nobutaka@nims.go.jp
Fax: +81-29-859-2475
Tel: +81-29-860-4774

b Graduate School of Natural Science and Technology, Okayama University, 3-1-1, Tsushima, Kita-ku, Okayama, Japan

c Department of Metallurgy and Ceramics Science, Tokyo Institute of Technology, O-okayama 2-12-1, Meguro-ku, Tokyo, Japan

d Nanotechnology Innovation Center, National Institute for Materials Science, 1-2-1 Sengen, Tsukuba, Ibaraki, Japan

e Advanced Nano-Characterization Center, National Institute for Materials Science, 3-13 Sakura, Tsukuba, Ibaraki, Japan

Abstract

Silicate–collagen hybrid fibril sheets were obtained using 3-glycidoxypropyl trimethoxysilane (GPTMS) as a novel cross-linker for type I collagen fibrils. BMP-2 was introduced in the hybrid sheets to stimulate cell proliferation and differentiation. Scanning electron microscopy images indicated that the resultant sheets were nanofibrous and porous. Transmission electron microscopy images showed that each hybrid fibril maintained a typical periodic unit of 60–70 nm. Compared to fibrils cross-linked by a conventional carbodiimide agent, the present hybrid fibrils were much more resistant against type I collagenase degradation. When soaked in BMP-2 solution, the hybrid sheets easily adsorbed BMP-2 and exhibited sustained release for BMP-2. Osteoblast-like MC3T3-E1 cells were well attached to and proliferated on the hybrid sheets. The proliferation and differentiation of these cells were enhanced to form collagen fibrils due to BMP-2 release.

Graphical abstract: Fabrication, microstructure, and BMP-2 delivery of novel biodegradable and biocompatible silicate–collagen hybrid fibril sheets

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Article information

DOI
https://doi.org/10.1039/C1JM10829H
Article type
Paper
Submitted
25 Feb 2011
Accepted
10 May 2011
First published
03 Jun 2011

J. Mater. Chem., 2011,21, 10942-10948

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Fabrication, microstructure, and BMP-2 delivery of novel biodegradable and biocompatible silicate–collagen hybrid fibril sheets

S. Chen, A. Osaka, T. Ikoma, H. Morita, J. Li, M. Takeguchi and N. Hanagata, J. Mater. Chem., 2011, 21, 10942 DOI: 10.1039/C1JM10829H

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