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Issue 29, 2010
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An injectable paste of calcium phosphate nanorods, functionalized with nucleic acids, for cell transfection and gene silencing

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Abstract

Calcium phosphate nanorods which are typically used as paste for bone substitution were functionalized by DNA or siRNA. The structure and morphology of the nanorods did not change by the functionalization as indicated by dynamic light scattering (DLS), scanning electron microscopy (SEM), and high-resolution transmission electron microscopy (HRTEM). With a load of nucleic acids of about 2.7 wt%, the nanorods were used for transfection with HeLa and T24 cells, and for gene silencing with HeLa-EGFP cells. Removal of the water by filtration gave an injectable paste with a content of nucleic acids of about 2 wt%, and a water content of 43 wt%. This leads to a bioactive paste as hard-tissue regeneration material.

Graphical abstract: An injectable paste of calcium phosphate nanorods, functionalized with nucleic acids, for cell transfection and gene silencing

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Publication details

The article was received on 20 Apr 2010, accepted on 27 May 2010 and first published on 23 Jun 2010


Article type: Paper
DOI: 10.1039/C0JM01130D
J. Mater. Chem., 2010,20, 6144-6148

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    An injectable paste of calcium phosphate nanorods, functionalized with nucleic acids, for cell transfection and gene silencing

    J. Klesing, S. Chernousova, A. Kovtun, S. Neumann, L. Ruiz, J. M. Gonzalez-Calbet, M. Vallet-Regi, R. Heumann and M. Epple, J. Mater. Chem., 2010, 20, 6144
    DOI: 10.1039/C0JM01130D

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