Issue 25, 2010

Secondary structure of silaffin at interfaces and titania formation

Abstract

We report on the secondary structure of the recombinant silaffin protein, rSilC, at liquid–solid and air–solid interfaces with polyelectrolyte layer-by-layer (LbL) films serving as templates to mediate protein adsorption. By exploiting in situ ATR-FTIR spectroscopy directly we revealed that the molecular layer of rSilC adsorbed on the LbL surface exhibits a random coil conformation in a hydrated state. In contrast, the partial transition into β-sheet state is observed when the protein is deposited by spin casting with fast water removal. Both forms of rSilC surface layers are capable of mineralization of titania nanostructures at ambient conditions. We suggest that the careful tailoring of the silaffin secondary structure both at interfaces and in solution with particular amino acid sequences capable of intra- and inter-molecular transformations is essential for directing the “bio-titaniamineralization resulting in nanoparticles to large microstructures.

Graphical abstract: Secondary structure of silaffin at interfaces and titania formation

Article information

Article type
Paper
Submitted
04 Mar 2010
Accepted
27 Apr 2010
First published
17 May 2010

J. Mater. Chem., 2010,20, 5242-5250

Secondary structure of silaffin at interfaces and titania formation

E. Kharlampieva, C. M. Jung, V. Kozlovskaya and V. V. Tsukruk, J. Mater. Chem., 2010, 20, 5242 DOI: 10.1039/C0JM00600A

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