Issue 17, 2010
From the journal:

Journal of Materials Chemistry

Photo-induced release of active plasmid from crosslinked nanoparticles: o-nitrobenzyl/methacrylate functionalized polyethyleneimine†

Moon Suk Kim,*ab   Jeffrey Gruneich,a   Huiyan Jinga  and  Scott L. Diamond*a  

* Corresponding authors

a Institute for Medicine and Engineering, Departments of Chemical and Biomolecular Engineering, 1024 Vagelos Research Laboratory, University of Pennsylvania Philadelphia, PA, USA
E-mail: sld@seas.upenn.edu
Fax: +1-215-573-7227
Tel: +1-215-573-5702

b Department of Molecular Science and Technology, Ajou University, Suwon, Korea
E-mail: moonskim@ajou.ac.kr
Fax: +82-31-219-3931
Tel: +82-31-219-2608

Abstract

To facilitate DNA packaging and photolytic release, o-nitrobenzyl and methacrylate functionalized PEI (P10A) was synthesized for condensing DNA into nanoparticles as small as 160 nm after radical polymerization with initiator. The gene expression following delivery of uncross-linked P10A/DNA was unchanged by photoirradiation of cells. However, exposure to photo-irradiation caused a 3-fold increase in gene expression in cells transfected with cross-linked P10A/DNA. These polyplexes were designed to mimic viral particles that condense and protect DNA while allowing subsequent triggered release of DNA.

Graphical abstract: Photo-induced release of active plasmid from crosslinked nanoparticles: o-nitrobenzyl/methacrylate functionalized polyethyleneimine

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Article information

DOI
https://doi.org/10.1039/B922613C
Article type
Paper
Submitted
29 Oct 2009
Accepted
03 Feb 2010
First published
04 Mar 2010

J. Mater. Chem., 2010,20, 3396-3403

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Photo-induced release of active plasmid from crosslinked nanoparticles: o-nitrobenzyl/methacrylate functionalized polyethyleneimine

M. S. Kim, J. Gruneich, H. Jing and S. L. Diamond, J. Mater. Chem., 2010, 20, 3396 DOI: 10.1039/B922613C

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