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Issue 6, 2012
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Simultaneous and ultra-sensitive quantification of multiple peptides by using europium chelate labeling and capillary electrophoresis-inductively coupled plasma mass spectrometry

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Abstract

More and more efforts are endeavored in the ultra-sensitive quantification of peptides and proteins, because many important peptides and proteins are present at ultra-low levels and only the quantity of them or the changes in their abundance really reflects the status and changes of a biological system. We herein report a novel ultra-sensitive method for the simultaneous quantification of three β-casomorphins (β-CMs) based on the Eu3+ diethylenetriamine-N,N,N′,N′′,N′′-pentaacetic acid labeling and capillary electrophoresis with on-line inductively coupled plasma mass spectrometry detection (CE-ICP-MS). The europium-tagged β-CMs were effectively separated by capillary electrophoresis and were sensitively determined by inductively coupled plasma mass spectrometry. Based on the europium-tagging and CE-ICP-MS, three β-CMs were baseline separated and detected within 15 min with an extremely low detection limit of 1.79–2.13 amol (1.79–2.13 × 10−11 mol L−1 for 100 nL of sample injection) and a RSD (n = 4) < 6%. The proposed method has been successfully used to detect β-CMs in real cheese samples with a recovery of 85–103%, suggesting that our method is sensitive and reliable. The success of the present method provides a new possibility for biological assays and clinical diagnoses and a new potential for simultaneous detection of low-abundant proteins or multiple biomarkers.

Graphical abstract: Simultaneous and ultra-sensitive quantification of multiple peptides by using europium chelate labeling and capillary electrophoresis-inductively coupled plasma mass spectrometry

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Supplementary files

Article information


Submitted
22 Feb 2012
Accepted
03 Apr 2012
First published
04 Apr 2012

J. Anal. At. Spectrom., 2012,27, 946-951
Article type
Paper

Simultaneous and ultra-sensitive quantification of multiple peptides by using europium chelate labeling and capillary electrophoresis-inductively coupled plasma mass spectrometry

M. W. Yang, Z. W. Wang, L. Fang, J. P. Zheng, L. J. Xu and F. F. Fu, J. Anal. At. Spectrom., 2012, 27, 946
DOI: 10.1039/C2JA30065F

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