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Issue 8, 2012
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Trace elements in human follicular fluid: development of a sensitive multielement ICP-MS method for use in biomonitoring studies

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Abstract

Little information is available in the literature regarding trace element analysis of human follicular fluid. Most peer-reviewed publications offer few details about sample preparation, method development, or method validation. We have attempted to fill this gap in current knowledge by providing comprehensive details of our method development and validation process for the measurement of 24 trace elements (As, Ba, Be, Cd, Co, Cr, Cs, Cu, Hg, Mo, Mg, Mn, Pb, Pt, Rb, Se, Sb, Sn, Sr, Tl, U, V, W, Zn) in follicular fluid. A number of different sample preparation procedures were explored, with a goal of optimizing method detection limits (MDLs), for both quadrupole-inductively coupled plasma-mass spectrometry (Q-ICP-MS) and sector field (SF)-ICP-MS. Human follicular fluid samples (n = 72) were obtained from 64 women undergoing in vitro fertilization (IVF) treatment, and were successfully analyzed for 24 trace elements using SF-ICP-MS.

Graphical abstract: Trace elements in human follicular fluid: development of a sensitive multielement ICP-MS method for use in biomonitoring studies

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Publication details

The article was received on 13 Feb 2012, accepted on 24 Apr 2012 and first published on 29 May 2012


Article type: Paper
DOI: 10.1039/C2JA30053B
Citation: J. Anal. At. Spectrom., 2012,27, 1245-1253

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    Trace elements in human follicular fluid: development of a sensitive multielement ICP-MS method for use in biomonitoring studies

    P. C. Kruger, M. S. Bloom, J. G. Arnason, C. D. Palmer, V. Y. Fujimoto and P. J. Parsons, J. Anal. At. Spectrom., 2012, 27, 1245
    DOI: 10.1039/C2JA30053B

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