Issue 6, 2011

A dual-labelling strategy for integrated ICPMS and LIF for the determination of peptides

Abstract

In this proof-of-concept study, a novel dual-labelling strategy for conjugating –SH and –NH2 of a peptide was developed, in which an element tag (1,4,7,10-tetraazacyclododecane-1,4,7-trisacetic acid-10-maleimidoethylacetamide loaded with europium) and a fluorescent tag (fluorescein isothiocycanate) were employed. Its feasibility was demonstrated using HPLC-UV/ESI-MS for evaluating labelling-efficiency and HPLC-ICPMS with 153Eu isotope dilution as well as CE-LIF for the determination of peptides.

Graphical abstract: A dual-labelling strategy for integrated ICPMS and LIF for the determination of peptides

Supplementary files

Article information

Article type
Communication
Submitted
06 Jan 2011
Accepted
18 Mar 2011
First published
01 Apr 2011

J. Anal. At. Spectrom., 2011,26, 1175-1177

A dual-labelling strategy for integrated ICPMS and LIF for the determination of peptides

Z. Zhang, X. Yan, M. Xu, L. Yang and Q. Wang, J. Anal. At. Spectrom., 2011, 26, 1175 DOI: 10.1039/C1JA00010A

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