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Issue 3, 1989
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Speciation of arsenic in urine using high-performance liquid chromatography with inductively coupled plasma mass spectrometric detection

Abstract

High-performance liquid chromatography (HPLC) has been coupled with inductively coupled plasma mass spectrometry (ICP-MS) for the speciation of four arsenic compounds commonly found in urine. Arsenite (As3+), arsenate (As5+), dimethylarsinate (DMA) and monomethylarsonate (MMA) were separated via anion-exchange HPLC and detected on-line using ICP-MS. The absolute detection limits ranged from 20 to 91 pg of arsenic in aqueous media and 36–96 pg in urine. The four species were determined in two freeze-dried urine standards using the method of standard additions. The total of the four species was found to be within 13% of the average reported total arsenic concentration for both freeze-dried urine standards containing elevated levels of As5+. The relative standard deviation of peak-height measurements was less than 10% for each of the four species in urine. The determination of As3+ is complicated by the presence of an interfering peak, which is believed to arise from the co-elution of chlorine-containing species and subsequent formation of 40Ar35CI+ ions (m/z= 75).

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Article information


J. Anal. At. Spectrom., 1989,4, 279-284
Article type
Paper

Speciation of arsenic in urine using high-performance liquid chromatography with inductively coupled plasma mass spectrometric detection

D. Heitkemper, J. Creed, J. Caruso and F. L. Fricke, J. Anal. At. Spectrom., 1989, 4, 279 DOI: 10.1039/JA9890400279

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