Jump to main content
Jump to site search

Issue 12, 2012
Previous Article Next Article

Sympathetic neurons modulate the beat rate of pluripotent cell-derived cardiomyocytes in vitro

Author affiliations

Abstract

Although stem cell-derived cardiomyocytes have great potential for the therapy of heart failure, it is unclear whether their function after grafting can be controlled by the host sympathetic nervous system, a component of the autonomic nervous system (ANS). Here we demonstrate the formation of functional connections between rat sympathetic superior cervical ganglion (SCG) neurons and pluripotent (P19.CL6) cell-derived cardiomyocytes (P19CMs) in compartmentalized co-culture, achieved using photolithographic microfabrication techniques. Formation of synapses between sympathetic neurons and P19CMs was confirmed by immunostaining with antibodies against β-3 tubulin, synapsin I and cardiac troponin-I. Changes in the beat rate of P19CMs were triggered after electrical stimulation of the co-cultured SCG neurons, and were affected by the pulse frequency of the electrical stimulation. Such changes in the beat rate were prevented when propranolol, a β-adrenoreceptor antagonist, was added to the culture medium. These results suggest that the beat rate of differentiated cardiomyocytes can be modulated by electrical stimulation of connected sympathetic neurons.

Graphical abstract: Sympathetic neurons modulate the beat rate of pluripotent cell-derived cardiomyocytes in vitro

Back to tab navigation

Article information


Submitted
16 Mar 2012
Accepted
26 Sep 2012
First published
01 Oct 2012

Integr. Biol., 2012,4, 1532-1539
Article type
Technical Innovation

Search articles by author

Spotlight

Advertisements