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Issue 10, 2009
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Microfluidic sample preparation: cell lysis and nucleic acidpurification

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Due to the lack of development in the area of sample preparation, few complete lab-on-a-chip systems have appeared in recent years that can deal with raw samples. Cell lysis and nucleic acid extraction systems are sufficiently complex even before adding the complexity of an analysis system. In this review, a variety of microfluidic sample preparation methods are discussed and evaluated. Microsystems for cell lysis are discussed by grouping them into categories based on their lysis mechanisms: mechanical, chemical, thermal or electrical. We classify the nucleic acidpurification techniques according to the mechanism that links nucleic acids to substrates: silica-based surface affinity, electrostatic interaction, nanoporous membrane filtration, and functionalized microparticles. The techniques for microfluidic cell lysis and nucleic acidpurification are compared based on the ease of microfabrication and integration, and sample flexibility. These assessments can help us determine the appropriate sample preparation technique for generating a true lab-on-a-chip.

Graphical abstract: Microfluidic sample preparation: cell lysis and nucleic acidpurification

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Publication details

The article was received on 24 Mar 2009, accepted on 10 Aug 2009 and first published on 25 Aug 2009

Article type: Critical Review
DOI: 10.1039/B905844C
Citation: Integr. Biol., 2009,1, 574-586

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