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Issue 20, 2020
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Non-photosynthetic CO2 bio-mitigation by Escherichia coli harbouring CBB genes

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Abstract

To alleviate carbon dioxide (CO2) emission, alternative approaches have been considered, such as employing microorganisms capable of CO2-fixation using their own metabolic pathways. In this study, we introduced a synthetic CO2-fixation pathway into the industrial platform microorganism Escherichia coli to achieve immediate CO2 mitigation with a non-photosynthetic reaction. Specifically, the whole gene clusters (cbbI and cbbII operons) involved in the Calvin–Benson–Bassham (CBB) pathway in Rhodobacter sphaeroides (rCBB) were expressed heterogeneously in E. coli under a restrictive carbon source and dark conditions. The resulting strain with the whole rCBB was able to exhibit a higher level of endogenous CO2 recycling compared to the control strain. In addition, the electron requirements from the cathode and redox cofactors were increased following the activation of the CO2-fixation pathway without light irradiation. Exogenous CO2 from yeast fermentation was also mitigated through a simple gas-phase connection with the E. coli/rCBB culture system. This bacterial CO2 mitigation strategy has great potential to alleviate greenhouse gas emissions through biological processes to produce carbon neutral biofuels and value-added chemicals.

Graphical abstract: Non-photosynthetic CO2 bio-mitigation by Escherichia coli harbouring CBB genes

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Supplementary files

Article information


Submitted
29 May 2020
Accepted
02 Sep 2020
First published
03 Sep 2020

Green Chem., 2020,22, 6889-6896
Article type
Paper

Non-photosynthetic CO2 bio-mitigation by Escherichia coli harbouring CBB genes

S. Y. Lee, Y. S. Kim, W. Shin, J. Yu, J. Lee, S. Lee, Y. Kim and J. Min, Green Chem., 2020, 22, 6889
DOI: 10.1039/D0GC01820A

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