Issue 8, 2019

One-step preparation of phosphate-rich carbonaceous spheres via a hydrothermal approach for phosphopeptide analysis

Abstract

Carbonaceous materials with different morphologies are attracting more and more attention in many fields due to their excellent performance. Various methods have been developed to prepare carbonaceous materials with different functions. In this work, we developed a “green” strategy to prepare phosphate-rich carbonaceous spheres via a one-step mild hydrothermal carbonization (HTC) process using glucose and vinylphosphonic acid (VPA) as precursors. Water was the sole solvent employed in this synthesis process, which is environmentally friendly. To augment the content of phosphate groups on the surfaces of carbonaceous spheres, water-soluble 4,4′-azobis(4-cyanovaleric acid) was added to the HTC system as a thermal initiator. The size of the obtained carbonaceous spheres could reach 1–8 μm. Following chelation with titanium ions (Ti4+), the carbonaceous spheres were applied as an immobilized metal affinity chromatography (IMAC) material; the Ti4+ content of carbonaceous spheres reached 2.685% (wt%). This synthetic process for Ti4+-IMAC carbonaceous spheres was facile and time-saving, avoiding the tedious and complex process for traditional Ti4+-IMAC materials. Furthermore, the Ti4+-IMAC carbonaceous spheres exhibited satisfactory enrichment ability for phosphopeptides in the digest of β-casein and could enrich the low-abundance phosphopeptides in human serum, indicating a great potential use in phosphopeptide analysis.

Graphical abstract: One-step preparation of phosphate-rich carbonaceous spheres via a hydrothermal approach for phosphopeptide analysis

Supplementary files

Article information

Article type
Paper
Submitted
27 Nov 2018
Accepted
06 Mar 2019
First published
06 Mar 2019

Green Chem., 2019,21, 2052-2060

One-step preparation of phosphate-rich carbonaceous spheres via a hydrothermal approach for phosphopeptide analysis

H. Zhang, X. Li, S. Ma, J. Ou, Y. Wei and M. Ye, Green Chem., 2019, 21, 2052 DOI: 10.1039/C8GC03706J

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements