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Issue 6, 2016
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Biotechnological production of acetoin, a bio-based platform chemical, from a lignocellulosic resource by metabolically engineered Enterobacter cloacae

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Abstract

Acetoin (AC) is regarded as one of the top potential sugar-derived chemical building blocks that can be used as food additives, precursors in chemical synthesis, and plant growth promoting molecules. In this study, a low-cost lignocellulosic resource of pretreated corn stover was used as a carbon source to produce AC. After redirecting the metabolic flux, fine tuning reducing power, and eliminating carbon catabolite repression in Enterobacter cloacae SDM, a systematically engineered strain SDM 53 was constructed, which is able to utilize glucose and xylose efficiently and simultaneously. Using fed-batch fermentation of SDM 53, 45.6 g L−1 AC was produced at a rate of 1.52 g L−1 h−1 using the lignocellulosic hydrolysate. Biotechnological synthesis of AC has various advantages such as being sustainable and environment-friendly. With its desirable properties, the engineered strain SDM 53 may be a potential choice for the industrial production of AC.

Graphical abstract: Biotechnological production of acetoin, a bio-based platform chemical, from a lignocellulosic resource by metabolically engineered Enterobacter cloacae

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Publication details

The article was received on 18 Jul 2015, accepted on 21 Oct 2015 and first published on 21 Oct 2015


Article type: Paper
DOI: 10.1039/C5GC01638J
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Green Chem., 2016,18, 1560-1570

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    Biotechnological production of acetoin, a bio-based platform chemical, from a lignocellulosic resource by metabolically engineered Enterobacter cloacae

    L. Zhang, Q. Liu, Y. Ge, L. Li, C. Gao, P. Xu and C. Ma, Green Chem., 2016, 18, 1560
    DOI: 10.1039/C5GC01638J

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