Jump to main content
Jump to site search

Issue 2, 2015
Previous Article Next Article

Breaking dormancy: an energy-efficient means of recovering astaxanthin from microalgae

Author affiliations

Abstract

Haematococcus pluvialis, in the dormant aplanospore (cyst) status after 30 d of cultivation, accumulates high levels of a superpotent antioxidant, astaxanthin, which has been demonstrated to have enormous therapeutic benefits. However, owing to the robust structure of its trilayered cell wall, the recovery of astaxanthin from the cyst cells remains an energy-intensive process. In the present study, a novel strategy utilizing a short-period germination based on the natural life cycle of H. pluvialis was developed as an energy-efficient pretreatment for the extraction of astaxanthin using ionic liquids (ILs) as green solvents. The germination resulted in damage and deconstruction of the cyst cell wall, and thereby facilitated the extraction of astaxanthin by ILs at room temperature. By this natural pretreatment with 1-ethyl-3-methylimidazolium ethylsulfate for a very short reaction time of 1 min, a high astaxanthin yield of 19.5 pg per cell was obtained, which was about 82% of a conventional volatile organic solvent extraction by strong, 30 000 psi French-pressure-cell homogenization. The maximal astaxanthin-extraction yield from H. pluvialis cells was observed for 12–18 h germination. The germination rate furthermore could be improved by manipulating the nutritional composition (especially the nitrate concentration) of the culture medium. In light of these results, it can be posited that natural germination following the principles of green chemistry can be a uniquely simple method of robust microalgal cyst cell pretreatment and extraction of astaxanthin with room-temperature ILs.

Graphical abstract: Breaking dormancy: an energy-efficient means of recovering astaxanthin from microalgae

Back to tab navigation

Supplementary files

Article information


Submitted
24 Jul 2014
Accepted
18 Nov 2014
First published
18 Nov 2014

Green Chem., 2015,17, 1226-1234
Article type
Paper
Author version available

Breaking dormancy: an energy-efficient means of recovering astaxanthin from microalgae

R. Praveenkumar, K. Lee, J. Lee and Y. Oh, Green Chem., 2015, 17, 1226
DOI: 10.1039/C4GC01413H

Social activity

Search articles by author

Spotlight

Advertisements