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Issue 3, 2012
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Production and purification of an extracellular lipolytic enzyme using ionic liquid-based aqueous two-phase systems

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Abstract

The ability of ionic liquid-based aqueous two-phase systems (ATPS) to purify lipase produced by fermentation is here evaluated and compared against conventional PEG-based ATPS systems. Four ionic liquids, chosen after screening of a larger number of ionic liquids are evaluated, with the maximum purification and higher recovery being obtained for the systems based on [C8mim]Cl. It is shown that IL-based ATPS have a performance superior to PEG-based ATPS for the purification of this enzyme.

Graphical abstract: Production and purification of an extracellular lipolytic enzyme using ionic liquid-based aqueous two-phase systems

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Article information


Submitted
09 Nov 2011
Accepted
16 Dec 2011
First published
18 Jan 2012

Green Chem., 2012,14, 734-740
Article type
Paper

Production and purification of an extracellular lipolytic enzyme using ionic liquid-based aqueous two-phase systems

S. P. M. Ventura, R. L. F. de Barros, J. M. de Pinho Barbosa, C. M. F. Soares, Á. S. Lima and J. A. P. Coutinho, Green Chem., 2012, 14, 734
DOI: 10.1039/C2GC16428K

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