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Issue 1, 2021
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Effect-directed analysis and chemical identification of agonists of peroxisome proliferator-activated receptors in white button mushroom

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Abstract

Obesity has a serious effect on human health. It relates to metabolic syndrome, including the associated disorders such as type 2 diabetes, heart disease, stroke and hyperemia. The peroxisome proliferator-activated receptors (PPARs) are important receptors to control fat metabolism in the human body. Because of the safety concerns of synthetic drugs targeting PPARs, ligands from natural sources have drawn interest. Earlier, we have found high PPAR activities in extracts from Agaricus bisporus (white button mushroom, WBM). WBM contains a wide range of candidate compounds which could be agonists of PPARs. To identify which compounds are responsible for PPAR activation by WBM extracts, we used fractionation coupled to effect-directed analysis with reporter gene assays specific for all three PPARs for purification and LC/MS-TOF and NMR for compound identification in purified active fractions. Surprisingly, we identified the relatively common dietary fatty acid, linoleic acid, as the main ligand of PPARs in WBM. Possibly, the relatively low levels of linoleic acid in WBM are sufficient and instrumental in inducing its anti-obesogenic effects, avoiding high energy intake and negative health effects associated with high levels of linoleic acid consumption. However, it could not be excluded that a minor relatively potent compound contributes towards PPAR activation, while the anti-obesity effects of WBM may be further enhanced by receptor expression modulating compounds or compounds with completely PPAR unrelated modes of action.

Graphical abstract: Effect-directed analysis and chemical identification of agonists of peroxisome proliferator-activated receptors in white button mushroom

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Supplementary files

Article information


Submitted
06 Aug 2020
Accepted
30 Oct 2020
First published
07 Dec 2020

This article is Open Access

Food Funct., 2021,12, 133-143
Article type
Paper

Effect-directed analysis and chemical identification of agonists of peroxisome proliferator-activated receptors in white button mushroom

L. Pham Ngoc, R. Helmus, A. W. Ehlers, K. Swart, H. Besselink, E. de Rijke, H. Dang Thi Cam, A. Brouwer and B. van der Burg, Food Funct., 2021, 12, 133
DOI: 10.1039/D0FO02071K

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