Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.


Issue 6, 2020
Previous Article Next Article

The ameliorative effect of Lactobacillus plantarum-12 on DSS-induced murine colitis

Author affiliations

Abstract

Some strains of lactobacilli can exert beneficial effects on a host when ingested in an adequate dose, such as immunoregulation and anti-inflammatory activities. In this study, the survival abilities under simulated gastrointestinal conditions, adhesion abilities on HT-29 cell monolayers, and hemolytic activities of four Lactobacillus plantarum strains were assessed. Among the four strains, L. plantarum-12 showed the higher survival rate under simulated gastrointestinal conditions and adhesion index on the HT-29 cell monolayers, exhibited γ-haemolytic activity and had no biological amine producing ability. L. plantarum-12 was administered to dextran sodium sulfate (DSS)-induced ulcerative colitis (UC) Balb/c mice by oral gavage for 10 days. It was observed that the UC Balb/c mice showed symptoms of colonic atrophy, intestinal histopathological change, gut microbial disturbance, and pro-inflammatory cytokine expression. L. plantarum-12 administration remarkably attenuated DSS-induced UC in mice. L. plantarum-12 administration could restore gut microbiota by increasing beneficial bacteria such as Lactobacillus and decreasing intestinal pathogenic bacteria like Proteobacteria. L. plantarum-12 administration could improve immunity via activating the janus kinase-signal transducer and the activator of the transcription (JAK-STAT) pathway and up-regulating adenosine deaminase (ADA) and interferon-induced protein with tetratricopeptide repeats 1 protein (IFIT1), and enforce the intestinal barrier function by up-regulating mucin 2 (MUC2) protein expression. In conclusion, L. plantarum-12 could attenuate DSS-induced UC in Balb/c mice by ameliorating intestinal inflammation, and restoring the disturbed gut microbiota. L. plantarum-12 could be used as promising probiotics to ameliorate colitis.

Graphical abstract: The ameliorative effect of Lactobacillus plantarum-12 on DSS-induced murine colitis

Back to tab navigation

Article information


Submitted
02 Jan 2020
Accepted
29 Apr 2020
First published
05 May 2020

Food Funct., 2020,11, 5205-5222
Article type
Paper

The ameliorative effect of Lactobacillus plantarum-12 on DSS-induced murine colitis

M. Sun, Y. Liu, Y. Song, Y. Gao, F. Zhao, Y. Luo, F. Qian, G. Mu and Y. Tuo, Food Funct., 2020, 11, 5205
DOI: 10.1039/D0FO00007H

Social activity

Search articles by author

Spotlight

Advertisements